BIOCHEMICAL STUDIES ON TOTAL PROTEIN, CARBOHYDRATE AND LIPIDS CONTENT LEVEL DURING THE INFECTION BY FUNGI WHITE MUSCARDINE DISEASE OF SILKWORM, BOMBYX MORI L. R. Nirupama*

The changes of protein, carbohydrate and lipids (pcl) metabolism were studied in haemolymph and whole larval tissues during the 5th instar larval development on 1st, 2nd, 3rd, 4th and 5th day after inoculation with fungi Beauveria bassiana (white muscardine) of conidial suspension,. The concentration level of pcl showed a similar trend of increasing in multivoltine and bivoltine silkworm breeds of control batches and reducing level was noticed in the infected batches, due to effect of fungal infection in both the breeds. The progressive concentration was found to be day by day reach peak level in healthy/control breeds, the bio-molecules are correlated to the degree of their absorption, inter-conversion and utilization in control batches. The biochemical results presumed on the basis of these major changes in protein, carbohydrate and lipid content level in infected batches causes for high toxicity of the silkworm larvae showing significant variation in their concentration. Two different varieties of silkworm breeds multivoltine 2000H and bivoltine CSR6 were utilized and bio-physiological status were discussed in relation to the 5th instar larvae under inoculated and control conditions.